Pair Name	Icariin, Gemcitabine
Disease Info	[ICD-11: 2C94]	Bladder cancer		Investigative
Biological Phenomena	Induction-->Blockade of cell cycle in G0/G1 phase
Gene Regulation	Down-regulation	Expression	BCL2	hsa596
	Down-regulation	Expression	BCL-xL	hsa598
	Down-regulation	Expression	BIRC5	hsa332
	Down-regulation	Expression	CASP3	hsa836
	Down-regulation	Activity	NFKB1	hsa4790
	Up-regulation	Expression	PARP1	hsa142
In Vitro Model	GBC-SD	Gallbladder carcinoma	Homo sapiens (Human)	CVCL_6903
	SGC-996	Gallbladder carcinoma	Homo sapiens (Human)	CVCL_M737
In Vivo Model	1×10⁵ GBC-SD cells were injected subcutaneously into the right and left abdominal regions with a gauge needle.
Result	Icariin, by suppressing NF-κB activity, exerts antitumor activity, and potentiates the antitumor activity of gemcitabine in gallbladder cancer. Combined administration of gemcitabine and icariin may offer a better therapeutic option for the patients with gallbladder cancer.